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Year : 2022  |  Volume : 9  |  Issue : 1  |  Page : 10-15

Sperm DNA fragmentation and reproductive outcomes

1 Department of Obstetrics and Gynaecology, Safdarjung Hospital and VMMC, New Delhi, India
2 Paschim Vihar, New Delhi, India

Correspondence Address:
Bindu Bajaj
Department of Obstetrics and Gynaecology, Safdarjung Hospital and VMMC, New Delhi
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/fsr.fsr_49_21

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In the last few years, male infertility is increasingly catching the attention of researchers. The impact of spermatozoal factors on the fertilization and early embryo development is being deciphered. An association of sperm aneuploidy status in unexplained recurrent pregnancy loss has been observed. Sperm DNA damage or the sperm DNA fragmentation (sDF) is now measurable as DNA fragmentation index. The methods of evaluating sDF are many and they differ in their technique and paraphernalia needed to perform them. The most common methods are SCSA, TUNEL, SCD, COMET. TUNEL utilizes tagging of DNA in places where there is a break and then quantifying them. SCD is based on the principal of susceptibility of fragmented DNA to acid denaturation. COMET assay involves variable movement of intact and fragmented DNA under the influence of electric field. There are different methods to perform each of the previous mentioned tests though the principle remains the same. Each laboratory performing these tests needs to standardize them and should have their own cutoffs, which should relate to the procedure [intrauterine insemination/in vitro fertilization (IVF)/intracytoplasmic sperm injection] being performed. The role of sDF is slowly getting defined in recurrent implantation failures, unexplained infertility, varicocele, and IVF failures. Yet the jury is still out.

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