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Year : 2022  |  Volume : 9  |  Issue : 1  |  Page : 29-37

Sperm DNA fragmentation − can it be a routine?

Crea Conceptions Private Limited, Kattupakkam, Chennai, Tamil Nadu, India

Correspondence Address:
Sasikala Natarajamani
Crea Conceptions Private Limited, No 2/220, Second Floor, Mount Poonamallee High Road, Kattupakkam, Chennai 600056, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/fsr.fsr_41_21

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Aim: To assess the correlation between semen parameters and sperm DNA fragmentation (SDF) and to examine whether SDF can be recommended as a routine test along with semen analysis. Materials and methods: Retrospective analysis was conducted for 118 male infertile patients attending a fertility clinic obtained from the records between 2017 and 2019. Semen analysis and SDF were assessed according to World Health Organization − 2010 guidelines and sperm chromatin dispersion test, respectively. Patients were grouped based on their SDF scores as “low DNA fragmentation group (LDF)” if SDF ≤ 18% and as “high DNA fragmentation group (HDF)” if SDF > 18%. Mann–Whitney and Kruskal–Wallis tests were used to find the difference in the mean of semen parameters between SDF groups. Statistical analysis was carried out using STATA Version-14. Results: The mean value of sperm concentration, motility, and normal morphology was higher in LDF compared to HDF. Correlation analysis showed patients’ age to be positively associated with SDF (r = 0.125; P = 0.102). Sperm concentration, progressive motility, and normal morphology had a weak negative correlation with SDF. Around 63% and 69% of patients with normal morphology and normal motility respectively had high SDF. In the HDF category, nearly 42% had normal morphology, whereas a 66% demonstrated normal motility. The results of Kruskal–Wallis test for patients who had a treatment outcome (conception) showed that patients in LDF group had 1.5 times higher chance of “conception and live birth.” Conclusion: Men with normal semen parameters can still have a high level of SDF. SDF along with semen analysis can help assess the reproductive potential of a male better.

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